Macromolecular synthesis in cells infected by frog virus 3.
نویسندگان
چکیده
Previous evidence indicated that frog virus 3 (FV3), an icosahedral DNA virus, replicates exclusively in the cytoplasm. However, data presented here demonstrate that FV3 does not replicate in UV-irradiated or enuleated chicken embryo or BSC-1 cells and that virus-specific DNA synthesis is not initiated in such cells. Primary transcription was not detected in infected enucleated cells. These results demonstrate that a functional nucleus is essential for FV3 replication.
منابع مشابه
Macromolecular synthesis in cells infected by frog virus 3. VII. Transcriptional and post-transcriptional regulation of virus gene expression.
We have used improved techniques for separating individual species of RNA and protein to study the mechanisms that control gene expression by frog virus 3, a eucaryotic DNA virus. Forty-seven species of viral RNA and 35 viral polypeptide species were resolved by polyacrylamide gel electrophoresis. The relative molar ratios of virus-specific polypeptides synthesized at various times after infect...
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ورودعنوان ژورنال:
- Current topics in microbiology and immunology
دوره 116 شماره
صفحات -
تاریخ انتشار 1977